The group of 25 single males, including 14 Afghans and 11 Pakistanis, arrived at Manus Island early on Saturday, the Department of Immigration and Citizenship said in a statement.
They were accompanied by Australian Federal Police, immigration staff, interpreters, childrens services and medical staff.
The latest transfer brings the total number of asylum seekers on Manus Island to 155.
Labor reopened the Howard-government era facility as part of a tough offshore processing regime it embraced in August, following recommendations from the Houston expert panel on asylum seekers.
The centre is expected to accommodate about 600 people when it is completed.
Source: Sky News Australia